Restriction Enzymes
New England Biolabs, Inc. MlyI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.
New England Biolabs, Inc. HpyCH4III – 1250 units
A restriction endonuclease that recognizes the sequence AC_N^GT.
New England Biolabs, Inc. HpyCH4V – 500 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. MlyI – 5000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNNN_^.
New England Biolabs, Inc. HpyCH4III – 250 units
A restriction endonuclease that recognizes the sequence AC_N^GT.
New England Biolabs, Inc. MwoI – 500 units
A restriction endonuclease that recognizes the sequence GCNN_NNN^NNGC.
New England Biolabs, Inc. DpnII – 1000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. AsiSI – 2500 units
A restriction endonuclease that recognizes the sequence GCG_AT^CGC.
New England Biolabs, Inc. I-SceI – 2500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. HpaII – 2000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. BstNI – 3000 units
A restriction endonuclease that recognizes the sequence CC^W_GG.
New England Biolabs, Inc. SwaI – 2000 units
A restriction endonuclease that recognizes the sequence ATTT_^AAAT.
New England Biolabs, Inc. APE 1 – 1000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
New England Biolabs, Inc. BfaI – 2500 units
A restriction endonuclease that recognizes the sequence C^TA_G.
New England Biolabs, Inc. Nb.BbvCI – 5000 units
A restriction endonuclease that recognizes the sequence CCTCA_GC.